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Heat Sealing Instrumentation

Thermal Test Film


With thermosensitive layer, for checking the uniformity and reproducibility of your heat sealer’s heating block.

Thermosensitive colour-forming film; for evaluation of consistent temperature across a heating block; to be used in conjunction with roll fed or sheet fed heat sealing instruments.

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With thermosensitive layer, for checking the uniformity and reproducibility of your heat sealer’s heating block.

4titude®’s Thermal Test Film (TTF) can be used in conjunction with a roll fed (e.g. a4S Automatic Roll Heat Sealer) or sheet fed (e.g. 4s3 Semi-Automatic Sheet Heat Sealer) heat sealer to check the uniformity and reproducibility of the heat sealing block of the instrument.

The film can be used to effectively test the temperature of the heating block between 160 – 200 °C.


Code Description Sheets or Kits
4ti-0640 sheets (125 x 80 mm) 25 sheets
4ti-0641 Starter Kit, 10 sheets (125 x 80 mm) and 1 silicone pad 1 kit
4ti-0642 Roll Kit, 1 roll (1 m x 80 mm) and 1 silicone pad 1 kit



The Thermal Test Film has a thermosensitive colour-forming layer plus a protective layer, both attached to the base material.

Depending on the temperature applied to the film, a colour is produced in varying density and hue – giving a perfect image of heat distribution across the heating block of your heat sealer.

The colour varies according to dwell time and temperature. The shorter the duration, the paler and more blueish the colour. The longer the duration, the more saturated and reddish the colour. Please refer to the colour chart below.


Note: This colour chart is just an example based on the results of the tests performed in our laboratories. Before using the Thermal Test Film, please create a similar chart that is based on your actual measurement conditions



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    4s3™ Semi-Automatic Sheet Heat Sealer


    A semi-automatic sheet heat sealer which is compatible with a wide range of seals and plates

    Semi-automatic sheet heat sealer compatible with a wide range of seals and microplates, PCR and assay plates, as well as storage plates of differing designs and heights

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    The 4s3™ is a semi-automatic sheet heat sealer which is compatible with a wide range of seals and plates of differing designs and heights.

    With variable temperature and time settings, sealing conditions are easily optimised to produce a 100% seal, eliminating sample loss.

    Plate and seal are placed on the holder, the “Operate” button pressed and the drawer automatically closes. The sealing process is controlled by an electric mechanism. This unique feature eliminates operator variation, giving a consistent seal every time and reducing the damage caused to microplates by over-sealing.



    – Automatic sealing process for unmatched sealing consistency
    – Variable temperature and time settings for easy optimisation of sealing conditions
    – Simple, single button operation
    – Rapid heating element for fast start up
    – Real-time temperature display
    – Auto stand-by function to save energy
    – Auto switch off function for added safety
    – Compatible with a wide variety of consumables including Random Access Plates
    – RS-232 serial port enables full integration with robot automation systems
    – Full range of heat seals available including Random Access Heat Seals (96 individual seal spots on a removable backing)
    – Small footprint
    – Low temperature sealing for use with adhesive seals


    4s3™ Semi-Automatic Sheet Heat Sealer  
    Code Description Quantity
    4ti-0655 Includes: universal power supply, power cord, manual, standard plate support adapter (4ti-0615), 12 month parts and labour warranty 1


    4ti-0615* 4s3™ Plate Support Adapter, Standard; for skirted 96 and 384 well plates 1
    4ti-0616 4s3™ Plate Support Adapter, Roche 1536; for Roche 1536 well PCR plates 1
    4ti-0625 4s3™ Plate Support Adapter, PCR 96; for 96 well PCR plates 1
    4ti-0613 4s3™ Plate Support Adapter, Random Access, Low Profile; for low profile plates 1
    4ti-0614 4s3 Plate Support Adapter, Random Access, Standard Profile; for standard profile plates 1
    4ti-0602 4s3™ Weighted Sealing Platen; for holding down sealing sheets that have a tendency to curl black, set of 2 1
    4ti-0612 4s3™ Sealing Frame; for use with Plate Support Adapter, PCR 96 (4ti-0625) 1
    4ti-0655-12 Warranty extension, second and third year 1
    4ti-0655-113 Warranty extension, third year 1

    *These items are included with the 4s3™ heat sealer (Code 4ti-0655)


    Real Time Display

    The rapid heating element allows for a quick start up within minutes. The temperature is displayed in real time so that the heating up process can be monitored.


    Energy Saving Functions

    When the unit is left idle it automatically switches to standby mode, during which the temperature of the heating element is reduced to 60 °C to save energy. When the sealer is left idle for longer periods it switches off automatically for added safety. The times at which the unit switches into standby and switch off modes can be adjusted to suit the user’s needs.


    Adapters and Sealing Aids

    The 4s3™ employs a unique and cost saving adapter system. Deep well plates and most other skirted plates can be sealed on the standard plate carrier (4ti-0615, supplied with the unit). For all 96 well PCR plates an additional plate carrier (4ti-0625, optional extra) must be used. Both plate carriers can also be combined for easier handling of shallow well plates such as 384 well PCR plates.

    For better sealing of those films which have a tendency to curl, 4titude® offer sealing aids. A weighted platen (4ti-0602) for use with deep well blocks can be ordered separately and a sealing frame for use with all other plate designs is supplied when ordering the optional 4ti-0625 adapter.

    The sealer allows for sealing of Random Access Plates with the corresponding Random Access Heat Seals using the Random Access adapter (4ti-0613).


    RS-232 Port for Automation Integration

    The 4s3™ has an RS-232 port to enable it to be integrated into most automated systems.


    Plate Formats

    The following plate formats can be used with an appropriate holder:

    SBS standard polypropylene and polystyrene plates and deep well blocks
    PCR plates including skirted, semi-skirted and non-skirted formats
    Random Access PCR Plates



    4titude® a4S Automatic Roll Heat Sealer Introduction Video


    4s3™ – How to Seal Tube Strips and Plate Segments


    4s3™ – How to Seal a Deep Well Plate


    4s3™ – How to Seal Standard 96 Well Non-Skirted PCR & qPCR Plates


    4s3™ – How to Seal FrameStar® 96 Well Semi-Skirted PCR Plates



    4titude® a4S Automatic Roll Heat Sealer Documents

    – a4S Product Flyer.pdf

    – Heat Sealing Consumables Comparison & Instrument Compatibility Table.pdf


    4s3™ Operation in 6 Easy Steps!

    1) Set Temperature

    2) Set Time

    3) Load Plate & Seal

    4) Press Operate

    5) The Plate is Automatically Sealed

    6) Remove Sealed Plate


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      a4S Automatic Roll Heat Sealer


      Is manual sealing and peeling of your microplates limiting your throughput?

      The perfect solution for automated heat sealing of microplates, PCR plates, assay plates and storage plates used in medium to high-throughput or full automation applications

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      Unrivalled sealing performance and consistency.

      The main advantage of the a4S is that it does not require an air supply, which gives it the flexibility to be used on standard lab benches as a stand-alone unit, or integrated within a robotic set‑up. The sealer’s rapid heating element enables a fast start-up, and the stand-by function saves energy when the unit is not in use.

      The a4S sealer is compatible with a wide range of SBS footprint plates, including all PCR plate formats from 96 well to 1536 well, assay plates, deep well storage plates and microplates. Varying plate heights, from 3 mm to 60 mm can be accommodated with minimal adjustments of the instrument. Plates are presented in portrait format and the plate position on the shuttle allows good access for robotic integration.

      Rolls with a width of between 75-85 mm can be loaded in either of two roll positions, depending on roll length and available space, enabling the entire instrument to fit under the gantree of most liquid handling robots.

      The width and length of the applied seals are set to precisely cover an SBS footprint plate without interfering with plate stacking. The seal position can be adjusted to give an overhang at one end of the plate to facilitate ease of seal removal.


      a4S Automatic Roll Heat Sealer  
      Code Description Quantity
      4ti-0665 Includes: universal power supply, power cord, plate support adapters A (4ti-0665-2) and B (4ti-0665-3), 24 month parts and labour warranty 1


      4ti-0665-1* a4S Seal Loading Tool 1
      4ti-0665-2* a4S Plate Support Adapter A 1
      4ti-0665-3* a4S Plate Support Adapter B 1
      4ti-0665-4* a4S Roll Holder Set; includes spindle, clamping wheels, locking nuts, O-rings 1
      4ti-0665-5* a4S Vacuum Cups, Front; clear, set of 2 2
      4ti-0665-6* a4S Vacuum Cups, Back; black, set of 2 2
      4ti-0665-7 Plastic Tweezers 1
      4ti-0665-8 a4S Clear Plastic Roll Dust Cover; Please Note: only applicable for smaller rolls (product code 4ti-xxxx/80, /100, /122) 1
      4ti-0665-41 SiLA driver for a4S heat sealer 1 License
      4ti-0665-11 Warranty extension, third year (36 months total) 1
      4ti-0665-12 Warranty extension, third and fourth year (48 months total) 1
      4ti-0665-113 Warranty extension, fourth year 1


      *These items are included with the a4S heat sealer (Code 4ti-0665)


      Simple Installation

      – No need for compressed air – Installation is fast and flexible

      – 2 positions for different roll sizes – Increased flexibility

      – Optional roll cover – Seal protection for sensitive applications


      Easy Handling

      – Colour touch screen with intuitive user interface – Ease of use

      – Unlimited password protected protocols – 3 security levels save personalised and SOP set temperatures and times

      – Rapid heating – Fast start up time, block uniformity is maintained to ± 1 °C

      – Variable time/temperature controls – Adjustment of time and temperature settings enables the perfect seal to be established for any sealer friendly, high-quality plate type, e.g. all 4titude® plates

      – Up to 5,000 seals without manual intervention – True walk-away-system


      Reproducible Sealing

      – Reproducible sealing – Fixed pressure and accurate time and temperature controls ensure consistent sealing

      – High sealing pressure – Improved seal uniformity on the widest range of plates

      – Sealing cycle time of less than 15 sec – Time saving


      Highest Flexibility

      – Compatible with a wide range of plate types, from racked tubes to 1536 well PCR plates – Highest flexibility

      – Flexibility of seal material choice – 4titude® offers a wide range of sealing materials, with a variety of properties for use with the a4S

      – Compatible with gas permeable heat seals – No instrument modification necessary for maximum convenience


      Economic Efficiency

      – Competitive pricing – Save money without compromises

      – Auto standby mode – Saves energy and prolongs component life

      – 2 year warranty on parts and labour

      – Extended warranty and service contracts available – For peace of mind


      Plug and Play Robotic Integration

      All 4titude® instruments are compliant with SiLA standards for rapid integration of automated systems (www.sila-standard.org). This means they can be “plug and play” connected with other instruments, such as readers, robotic arms and liquid handlers, to give a custom automated system without the need to write expensive custom drivers. The a4S heat sealer is extremely versatile leaving you the freedom to expand and configure your systems such as adding robotic plate handling.

      – Full communication protocol available – Operate the instrument and record sealing conditions for each seal using the RS232 communication port

      – SiLA compatible – Quick and easy integration with other SiLA compliant devices using the SiLA driver (available at extra cost, please see ordering information).



      Heat sealing is the gold standard method for plate sealing. Not only does it minimise sample loss but also maximises sample security for PCR, qPCR, storage, and other applications.


      qPCR – Reduce evaporation and save money

      The optimised sealing performance of the a4S heat sealer prevents reagent volume loss by evaporation.

      Up to a fivefold reduction in the costs of sealing can be achieved when heat sealing with the a4S compared to using caps or adhesive seals – Reliable qPCR results and significant reagent savings.

      – Clear Heat Seal (4ti-0540) and Clear Weld Heat Seal Mark 2 (4ti-0573) are widely used for qPCR

      – Clear Weld Heat Seal Mark 2 is particularly designed for Hydrocyclers


      Sample Storage – Strongest resistance against organic solvents

      Essential requirements for long-term storage include maintaining sample integrity and minimising the risks of sample degradation.

      – e.g. Foil Heat Seal (4ti-0535), Pierce Heat Seal (4ti-0530), and DMSO Resistant Peel Heat Seal (4ti-0585) – These seals when applied using the a4S heat sealer demonstrate the highest performance in compound storage, drug screening and subsequent sample retrieval.

      – DMSO Resistant Peel Heat Seal – The first 100% DMSO resistant seal. Pure DMSO can be stored at room temperature for 12 months with this seal with no deterioration of the seal quality.


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