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FrameStar® 96 Well Semi-Skirted PCR Plate, ABI FastPlate Style

96 Well Semi-Skirted PCR Plate, ABI FastPlate Style

Accurate, Sensitive and Consistent for Real Time PCR

Low profile, 0.1 ml polypropylene wells, polycarbonate frame with upstand, cut corner A1, working volume: <100 µl, total well capacity: 200 µl; designed for use on ABI® Fast Block cyclers.

This semi-skirted low profile plate is recommended for use with ABI® Fast block thermal cyclers.

The low profile wells of this plate are shorter than “standard” profile wells, which decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems. Its skirt also allows for labelling or barcoding.

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Plate Features

  • Compatible with ABI® Fast block thermal cyclers

  • Recommended for low volume PCR

  • Rigid polycarbonate frame adds to the mechanical stability of the plate – as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results

  • Improved seal integrity due to thermal stability of frame

  • Compatible with standard multichannel pipettes

  • Ideal for use with robotic systems

  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity

  • Raised and colour differentiated grid reference to aid well and sample identification

  • Stackable

  • Available barcoded

  • Combi packs available (for 4ti-0910/C) with Q-Stick™ qPCR Seal (4ti-0565)

  • Standard plate comes with clear polypropylene wells with a clear polypropylene frame

  • Also available with white wells for optimum signal-to-noise ratio when using fluorescent based assays; and with frosted wells (see below)

Now Available – Frosted Wells for use with ABI®/LifeTechnologies® qPCR Instruments

While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflect of light in fluorescent based assays used in real-time PCR and qPCR. This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.

However, white well PCR plates may lead to software related problems with ABI®/Life Technologies® cyclers. For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.

In response to this, we at 4titude® have developed a frosted well variety of the FrameStar® FastPlate 96 well to offer ABI®/Life Technologies® instrument users the time and money-saving advantages of FrameStar®. And as the signal levels are directly comparable with ABI®/Life Technologies® plates, there’s no need for users to recalibrate their instruments.

Find these plates in the Order tab under product code 4ti-0912.

Frosted wells compared to clear wells


This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding.


Manufacturer Block Type Instrument
ABI® / LIFE TECHNOLOGIES / THERMO FISHER SCIENTIFIC Thermal Cyclers (96 well FAST block) GeneAmp® 9800 FAST | Verity FAST
qPCR Cyclers (96 well FAST block) StepOne Plus™ | 750 FAST | 7900 HT FAST | QuantStudio™ 5 / 6 / 7 / 12K | ViiA7™

Thermal Cyclers (96 well block) Labcycler



FrameStar® 96 Well Semi-Skirted PCR Plate, ABI FastPlate Style
Low profile, 0.1 ml polypropylene wells, polycarbonate frame with upstand, cut corner A1, working volume: <100 µl, total well capacity: 200 µl; designed for use on ABI® Fast Block cyclers

Code Description Plates/Case
4ti-0910/C clear wells, clear frame 50 plates
4ti-0910/C/10P clear wells, clear frame 10 plates
4ti-0911 white wells, clear frame 50 plates 
4ti-0912 frosted wells, clear frame 50 plates
4ti-0912/10P frosted wells, clear frame 10 plates 
Combi Pack Code Description Plates & Seals/Case
4ti-0910/C/0565 4ti-0910/C + Q-Stick™ qPCR Seal 50 plates + 50 seals


Product Info

Plate Width 127.76 ± 0.25 mm
Plate Depth 85.48 ± 0.25 mm
Plate Height 16.70 ± 0.25 mm
Well Depth 15.10 ± 0.10 mm
Well Diameter 5.50 ± 0.10 mm
Distance to centre of A1 from top edge 11.24 ± 0.25 mm
Distance to centre of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm