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FrameStar® 96 Well Semi-Skirted PCR Plate, ABI Style

96 Well Semi-Skirted PCR Plate, ABI Style

Accurate, Sensitive and Consistent for Real Time PCR

Standard profile, 0.2 ml polypropylene wells, polycarbonate frame, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; designed for use on all major cycles, including ABI® instruments with standard 96 well blocks; now also available as low binding plate with ultra-low DNA binding properties.

Specifically designed to be directly compatible with all major thermal cycles, this plate can be used directly in ABI® 96 well instruments without the need for any adapters.

The rigid FrameStar® 2-component design eliminates warping and distortion during PCR, making it ideal for use with robotic systems. The semi-skirt allows for labeling or barcoding for sample tracking.

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Plate Features

  • Cut corner at A12 – for a variety of plate with a cut corner at H1

  • Perfect for ABI® thermal cyclers & sequencers

  • Recommended for low volume PCR

  • Rigid polycarbonate frame adds to the mechanical stability of the plate – as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results

  • Improved seal integrity due to thermal stability of frame

  • Compatible with standard multichannel pipettes

  • Ideal for use with robotic systems

  • <0.2 ml (200 µl) working volume, 0.3 ml (300 µl) total well capacity

  • Raised and colour differentiated grid reference to aid well and sample identification

  • Available barcoded

  • 6 frame colour options for the clear well variety – purple, blue, clear, green, red, and black

  • Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays; and as a clear frame with frosted wells (see below)

Now Available – Low Binding Plates with Ultra-Low DNA Binding Properties

Sensitive applications such as Next Generation Sequencing sample preparation applications demand the highest quality consumables. Low binding polypropylene tubes allow for multiple incubation and transfer steps without sample loss.

Polypropylene (PP) is the best plastic material for PCR tubes as it is chemically inert, resistant to solvents, and well suited for injection moulding – allowing for production of thin-walled tubes for optimum PCR results.

DNA has been shown to bind to PP tubes especially at high ionic strength, despite the very hydrophobic nature of this material. Different PP polymers are used for the production of PCR consumables and as they differ in their characteristics including surface charges, they consequently bind DNA in varying amounts.

DNA binding to PP surfaces has typically only been an issue for reaction tubes and storage vessels but not for PCR/qPCR tubes. DNA sticking to tube walls is either released during denaturation steps and/or remains accessible for amplification.

Nevertheless, due to a progressing volume miniaturisation and with new technologies such as Next Generation Sequencing (NGS), PCR/qPCR tubes are more and more also recommended for other applications which may require ultra-low DNA binding.

Plates of this design with ultra-low DNA binding properties are now available to order under the product code 4ti-LB0770/C.

  • Low-binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics – No contamination or modification of the samples

  • Tested under a broad temperature range – Maximum DNA recovery after low temperature storage and high temperature incubation

  • No DNA loss during incubation steps and sample transfer in NGS sample prep and library construction – Ideally suited for sensitive applications with ultra-low DNA input

Now Available – Frosted Wells for use with ABI®/LifeTechnologies® qPCR Instruments

While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflect of light in fluorescent based assays used in real-time PCR and qPCR. This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.

However, white well PCR plates may lead to software related problems with ABI®/LifeTechnologies® cyclers. For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.

In response to this, we at 4titude® have developed a frosted well variety of the FrameStar® 96 Well Semi-Skirted Plate to offer ABI®/LifeTechnologies® instrument users the time and money-saving advantages of FrameStar®. And as the signal levels are directly comparable with ABI®/LifeTechnologies® plates, there’s no need for users to recalibrate their instruments.

Find these plates in the Order tab under product code 4ti-0772.

Frosted wells compared to clear wells

Now Available – Ethylene Oxide Treated Plates

Forensic applications demand the highest quality consumables. Avoiding external DNA contamination is of the utmost importance, and as a result high demands are placed upon manufacturers of products for use throughout the workflow.

At 4titude®, we have a stringently controlled cleanroom production facility certified, according to ISO 9001 and ISO 13485, for production of DNA and RNA free products. Following cleanroom production, 4titude®’s consumables for forensic use are packed into ethylene oxide permeable bags and then subjected to ethylene oxide sterilisation. This 2-step processing ensures degradation of amplifiable DNA.

Plates of this design for forensic use are now available to order under the product code 4ti-OX770C/SBC.


This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding.


Manufacturer Block Type Instrument
ABI® / LIFE TECHNOLOGIES / THERMO FISHER SCIENTIFIC Thermal Cyclers (96 well standard block) Verity | Proflex | Simpliamp | GeneAmp® 2700 / 2720 / 9600 / 9700
qPCR Cyclers (96 well standard block) 7000 | 7300 | 7500 | 7700 | 7900 HT | QuantStudio™ 3 / 5 / 6 / 7 / 12K | ViiA7™
Sequencers (96 well block) 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
AGILENT / STRATAGENE qPCR Cyclers (96 well block) Mx3000P™ | Mx3005P™ | Mx4000P™ |
ANALYTIK JENA / BIOMETRA Thermal Cyclers (96 well block) Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard / Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)
qPCR Cyclers (96 well block) qTOWER3 / G/ touch | Toptical
BIOER TECHNOLOGIES Thermal Cyclers (96 well block) Gene Touch 96
BIO-RAD Thermal Cyclers (96 well block) C1000 Touch | S1000 | iCycler™ | MyCycler™ | T100
qPCR Cyclers (96 well block) MyiQ™ | iCycler™ IQ / IQ 4 / IQ 5
BIO-RAD MJ RESEARCH Thermal Cyclers (96 well block) PCT100™ / 200™ / 220™ / 221™ / 225™ / 240™
qPCR Cyclers (96 well block) Chromo4™
CORBET RESEARCH Thermal Cyclers (96 well block) (Qiagen) Palm Cycler
EPPENDORF Thermal Cyclers (96 well block) MasterCycler® ep / ep gradient / Pro / Pro S / nexus / nexus gradient / nexus SX1 / nexus GSX1
qPCR Cyclers (96 well block) Mastercycler™ e realplex
PEQLAB / VWR Thermal Cyclers (96 well block) peqSTAR 96X
SENSOQUEST Thermal Cyclers (96 well block) Labcycler
TAKARA Thermal Cyclers (96 well block) Dice touch | Gradient

Thermal Cyclers (96 well block) Prime | PrimeG | Prime Elite | Prime Elite Satellite | PCRmax Alpha cycler 1 / 2/ 4 | TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene



FrameStar® 96 Well Semi-Skirted PCR Plate, ABI Style
Standard profile, 0.2 ml polypropylene wells, polycarbonate frame, cut corner H1, working volume: <200 µl, total well capacity: 300 µl

Code Description Plates/Case
4ti-0770 clear wells, purple frame 50 plates
4ti-0770/B clear wells, blue frame 50 plates
4ti-0770/C clear wells, clear frame 50 plates 
4ti-0770/C/10P clear wells, clear frame 10 plates
4ti-0770/G clear wells, green frame 50 plates 
4ti-0770/R clear wells, red frame 50 plates 
4ti-0770/X clear wells, black frame 50 plates 
4ti-0771 white wells, black frame 50 plates 
4ti-0772 frosted wells, clear frame 50 plates 
4ti-0772/10P frosted wells, clear frame 10 plates
4ti-OX770C/SBC 4ti-0770/C for forensic use, ethylene oxide treated, single barcoded 20 plates
4ti-LB0770/C 4ti-0770/C, with low DNA binding properties 50 plates
Lids Code Description Lids/Case
4ti-0289 FrameStar® 96 Lid, for use with the FrameStar® 96 Well Semi-Skirted Plates, ABI® Style range 50 lids


Product Info

Plate Width 124.26 ± 0.25 mm
Plate Depth 83.97 ± 0.25 mm
Plate Height 20.70 ± 0.25 mm
Well Depth 20.20 ± 0.10 mm
Well Diameter 5.46 ± 0.10 mm
Distance to centre of A1 from top edge 10.495 ± 0.25 mm
Distance to centre of A1 from left edge 12.63 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm