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FrameStar® 96 Well Skirted PCR Plate

96 Well Skirted PCR Plate

Accurate, Sensitive and Consistent for Real Time PCR

Low profile, 0.1 ml polypropylene wells, rigid polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 200 µl; now also available as low binding plate with ultra-low DNA binding properties.

The low profile wells of this plate are shorter than “standard” profile wells, which decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

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Plate Features

  • The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems. It adds stability of the plate – as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results

  • Recommended for low volume PCR

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results

  • Improved seal integrity due to thermal stability of frame

  • Compatible with standard multichannel pipettes

  • Ideal for use with robotic systems

  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity

  • 7 frame colour options for the clear well variety – purple, blue, clear, green, red, black, and white

  • Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays and as a black frame with black wells for non-PCR fluorescent applications

  • Now available with an even more rigid skirt for use with Perkin-Elmer® Sciclone, Beckman, Hamilton, and other automation systems – eliminates the robotic grip picking up more than one plate at a time (4ti-0960/RIG)

  • Clear Polystyrene Lid available for 4ti-0960/RIG

  • Raised and colour differentiated grid reference to aid well and sample identification

  • Stackable

  • Available barcoded

  • Combi packs available (for 4ti-0960) with Q-Stick™ qPCR Seal (4ti-0565)

Now Available – Low Binding Plates with Ultra-Low DNA Binding Properties

Sensitive applications such as Next Generation Sequencing sample preparation applications demand the highest quality consumables. Low binding polypropylene tubes allow for multiple incubation and transfer steps without sample loss.

Polypropylene (PP) is the best plastic material for PCR tubes as it is chemically inert, resistant to solvents, and well suited for injection moulding – allowing for production of thin-walled tubes for optimum PCR results.

DNA has been shown to bind to PP tubes especially at high ionic strength, despite the very hydrophobic nature of this material. Different PP polymers are used for the production of PCR consumables and as they differ in their characteristics including surface charges, they consequently bind DNA in varying amounts.

DNA binding to PP surfaces has typically only been an issue for reaction tubes and storage vessels but not for PCR/qPCR tubes. DNA sticking to tube walls is either released during denaturation steps and/or remains accessible for amplification.

Nevertheless, due to a progressing volume miniaturisation and with new technologies such as Next Generation Sequencing (NGS), PCR/qPCR tubes are more and more also recommended for other applications which may require ultra-low DNA binding.

Plates of this design with ultra-low DNA binding properties are now available to order under the product code 4ti-LB0960/RIG.

  • Low-binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics – No contamination or modification of the samples

  • Tested under a broad temperature range – Maximum DNA recovery after low temperature storage and high temperature incubation

  • No DNA loss during incubation steps and sample transfer in NGS sample prep and library construction – Ideally suited for sensitive applications with ultra-low DNA input

Now Available – Ethylene Oxide Treated Plates

Forensic applications demand the highest quality consumables. Avoiding external DNA contamination is of the utmost importance, and as a result high demands are placed upon manufacturers of products for use throughout the workflow.

At 4titude®, we have a stringently controlled cleanroom production facility certified, according to ISO 9001 and ISO 13485, for production of DNA and RNA free products. Following cleanroom production, 4titude®’s consumables for forensic use are packed into ethylene oxide permeable bags and then subjected to ethylene oxide sterilisation. This 2-step processing ensures degradation of amplifiable DNA. Read more here »

Plates of this design for forensic use are now available to order under the product code 4ti-OX960C/SBC.


This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding.


Manufacturer Block Type Instrument
AGILENT/STRATAGENE Thermal Cyclers (96 well block) Robocycler Gradient
qPCR Cyclers (96 well block) AriaMx
ANALYTIK JENA / BIOMETRA Thermal Cyclers (96 well block) Flexcycler2 | T1 Thermocycler | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard / Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)
BIO-RAD qPCR Cyclers (96 well block) C1000 Touch | S1000
Thermal Cyclers (96 well block) CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
BIO-RAD MJ RESEARCH Thermal Cyclers (96 well block) PCT100™ / 200™ / 220™ / 221™ / 225™ / 240™
qPCR Cyclers (96 well block) Chromo4™ | Opticon2™
EPPENDORF Thermal Cyclers (96 well block) MasterCycler® ep / ep gradient / Pro / Pro S / nexus / nexus gradient / nexus SX1 / nexus GSX1
qPCR Cyclers (96 well block) MasterCycler™ ep realplex
GE HEALTHCARE / AMERSHAM Sequencers (96 well block) MegaBACE™ 500 | MegaBACE™ 1000 mark 2
PEQLAB / VWR Thermal Cyclers (96 well block) peqSTAR 96X
SENSOQUEST Thermal Cyclers (96 well block) Labcycler

Thermal Cyclers (96 well block) TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
qPCR Cyclers (96 well block) Quantica



FrameStar® 96 Well Skirted PCR Plate
PP wells, PC frame, cut corner A24, working volume: 30 µl, total well capacity: 55 µl

Code Description Plates/Case
4ti-0960 clear wells, purple frame 50 plates
4ti-0960/B clear wells, blue frame 50 plates
4ti-0960/C clear wells, clear frame 50 plates
4ti-0960/G clear wells, green frame 50 plates
4ti-0960/R clear wells, red frame 50 plates
4ti-0960/X clear wells, black frame 50 plates
4ti-0960/W clear wells, white frame 50 plates
4ti-0961 white wells, black frame 50 plates
4ti-0966 black wells, black frame 50 plates
4ti-OX960C/SBC 4ti-0960/C for forensic use, ethylene oxide treated and single barcoded 20 plates 
4ti-0960/RIG 4ti-0960/X with extra rigid skirt 50 plates 
4ti-LB0960/RIG 4ti-0960/RIG, with low DNA binding properties 50 plates 

FrameStar® 96 Well Skirted PCR Plate

Combi Pack Code Description Plates and Seals/Case
4ti-0960/0565 4ti-0960 + Q-Stick™ qPCR Seal (4ti-0565) 50 plates + 50 seals
Lids Code Description Lids/Case
4ti-0287 FrameStar® 96 NGS Lid, for use with 4ti-0960/RIG 50 lids


Product Info

Plate Width 127.76 ± 0.25 mm
Plate Depth 85.48 ± 0.25 mm
Plate Height 16.10 ± 0.25 mm
Well Depth 15.10 ± 0.10 mm
Well Diameter 5.50 ± 0.10 mm
Distance to centre of A1 from top edge 11.24 ± 0.25 mm
Distance to centre of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm