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FrameStar® 96 Well Semi-Skirted PCR Plate With Upstand, ABI Style

FRAMESTAR
96 Well Semi-Skirted PCR Plate With Upstand, ABI Style

Accurate, Sensitive and Consistent for Real Time PCR

Standard profile, 0.2 ml polypropylene wells, polycarbonate frame with upstand, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; designed for use on ABI® instruments.

This ABI® Style PCR plate offers the benefits of our 2-component design to ABI® standard block users. This design combines the advantages of ultra-thin wall polypropylene tubes for optimum PCR results with a rigid polycarbonate skirt and deck for the highest thermal stability.

We recommend this semi-skirted plate for use with ABI® thermal cyclers and sequencers; it can be used directly with ABI® instruments with no adaptors necessary. The only case where this is not true is with the ABI Fast Block thermal cyclers; in which case using our FrameStar® FastPlate is recommended instead – found here.

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Plate Features

  • Ideal for use with ABI® thermal cyclers & sequencers

  • Recommended for low volume PCR

  • Rigid polycarbonate frame adds to the mechanical stability of the plate – as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results

  • Improved seal integrity due to thermal stability of frame

  • Compatible with standard multichannel pipettes

  • Ideal for use with robotic systems

  • <0.2 ml (200 µl) working volume, 0.3 ml (300 µl) total well capacity

  • 6 frame colour options for the clear well variety – purple, blue, clear, green, red, and black

  • Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays

  • Raised and colour differentiated grid reference to aid well and sample identification

  • Available barcoded

  • Combi packs available (for 4ti-0730/C) with Q-Stick™ qPCR Seal (4ti-0565)

Now Available – Ethylene Oxide Treated Plates

Forensic applications demand the highest quality consumables. Avoiding external DNA contamination is of the utmost importance, and as a result high demands are placed upon manufacturers of products for use throughout the workflow.

At 4titude®, we have a stringently controlled cleanroom production facility certified, according to ISO 9001 and ISO 13485, for production of DNA and RNA free products. Following cleanroom production, 4titude®’s consumables for forensic use are packed into ethylene oxide permeable bags and then subjected to ethylene oxide sterilisation. This 2-step processing ensures degradation of amplifiable DNA.

Plates of this design for forensic use are now available to order under the product code 4ti-OX730C/SBC.

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding.

Compatibility

Manufacturer Block Type Instrument
ABI® / LIFE TECHNOLOGIES / THERMO FISHER SCIENTIFIC Thermal Cyclers (96 well standard block) Verity | Proflex | Simpliamp | GeneAmp® 2700 / 2720 / 9600 / 9700
qPCR Cyclers (96 well standard block) 7000 | 7300 | 7500 | 7700 | 7900 HT | QuantStudio™ 3 / 5 / 6 / 7 / 12K | ViiA7™
Sequencers (96 well block) 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
AGILENT / STRATAGENE qPCR Cyclers (96 well block) Mx4000™
SENSOQUEST Thermal Cyclers (96 well block) Labcycler

 

Ordering

FrameStar® 96 Well Semi-Skirted PCR Plate With Upstand, ABI Style
Standard profile, 0.2 ml polypropylene wells, polycarbonate frame with upstand, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; designed for use on ABI® instruments

Code Description Plates/Case
4ti-0730 clear wells, purple frame 50 plates
4ti-0730/B clear wells, blue frame 50 plates
4ti-0730/C clear wells, clear frame 50 plates 
4ti-0730/C/10P clear wells, clear frame 10 plates
4ti-0730/G clear wells, green frame 50 plates 
4ti-0730/R clear wells, red frame 50 plates 
4ti-0730/X clear wells, black frame 50 plates 
4ti-0731 white wells, black frame 50 plates 
4ti-OX730C/SBC 4ti-0730/C for forensic use, ethylene oxide treated, single barcoded 20 plates
Combi Pack Code Description Plates & Seals/Case
4ti-0730/C/0565 4ti-0730/C + Q-Stick™ qPCR Seal (4ti-0565) 50 plates + 50 seals

 

Product Info

Plate Width 124.26 ± 0.25 mm
Plate Depth 83.97 ± 0.25 mm
Plate Height 23.20 ± 0.25 mm
Well Depth 20.20 ± 0.10 mm
Well Diameter 5.46 ± 0.10 mm
Distance to centre of A1 from top edge 10.495 ± 0.25 mm
Distance to centre of A1 from left edge 12.63 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm

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