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FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style High Sensitivity

FRAMESTAR
96 Well Semi-Skirted PCR Plate, Roche Style High Sensitivity

Accurate, Sensitive and Consistent for Real Time PCR

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays.

Our FrameStar® Roche Style plates are designed to achieve optimised assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block).

This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl. The wells are shorter than “standard” profile wells, which decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

The extra white wells allow for increased sensitivity in fluorescent assays.

The semi-skirted frame allows for labelling or barcoding.

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Plate Features

  • Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)

  • Recommended for low volume PCR

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results

  • Compatible with standard multichannel pipettes

  • Ideal for use with robotic systems

  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity

  • Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays

  • Raised and colour differentiated grid reference to aid well and sample identification

  • Available barcoded

  • Combi packs available with qPCR Seal (4ti-0560)

Application Data

Ct values of data run on a Roche LightCycler® 480, comparing 4titude FrameStar®Roche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates. The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct values.

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding.

Compatibility

Manufacturer Block Type Instrument
ROCHE qPCR Cyclers (96 well block) LC96 | LC480

 

Ordering

FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style High Sensitivity
Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays

Code Description Plates/Case
4ti-0954 extra white wells, clear frame 50 plates
     
Combi Pack Code Description Plates & Seals/Case
4ti-0954/0560 4ti-0954 + qPCR Seal (4ti-0560) 50 plates + 50 seals



Product Info

Plate Width 127.70 ± 0.25 mm
Plate Depth 85.48 ± 0.25 mm
Plate Height 15.60 ± 0.25 mm
Well Depth 15.10 ± 0.10 mm
Well Diameter 5.50 ± 0.10 mm
Distance to centre of A1 from top edge 11.24 ± 0.25 mm
Distance to centre of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm

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