96 Well Semi-Skirted PCR Plate, Roche Style

Product Code: 4ti-0955

96 well semi-skirted plates designed for use on Roche LightCycler® 480 (with 96 well block). Low profile wells, especially recommended for reaction volumes <20µl.

  • Virgin polypropylene; cut corner H12
  • <100µl working volume, 200µl total well capacity
  • Available barcoded on request

Key Features

Quality

  • Subject to a strict QC procedure which includes a visual check and leak testing of every tube
  • Molded from virgin polypropylene under ISO certified cleanroom conditions in our UK-based production facility
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
  • Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
  • Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
  • Maximum thermal conductivity for efficient heat transfer and precise thermal cycling

Frame

  • Alphanumeric grid reference to aid well and sample identification

Use

  • Optimized for use with the Roche LightCycler 480
  • Recommended for low volume PCR
  • Compatible with standard multichannel pipettes

Options

  • Available with white wells give optimum signal-to-noise ratio when using fluorescent-based assays
  • Combi packs available with qPCR Seal (0560)
  • Available barcoded upon request; learn more about our barcoding options
  • Available as a FrameStar® 2-component PCR plate with the same dimensions, but with the added benefits of a polycarbonate frame that eliminates plate distortion and warping during the PCR process, leading to less evaporation and sample loss

 

Specifications

Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 15.60 ± 0.10mm
Well depth 15.10 ± 0.10mm
Well diameter 5.50 ± 0.10mm
Total well capacity 0.2ml (200µl)
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm

 

Compatibility

Roche

qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

 

Ordering Information

Part Numbers

96 Well Semi-Skirted PCR Plate, Roche Style
Low profile, 0.1ml wells, polypropylene, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler 480
4ti-0955 white; 50 plates
 

Combi Packs
4ti-0955/0560 4ti-0955 + qPCR Seal (4ti-0560); 50 plates + 50 seals