Key Features
Quality
- Subject to a strict QC procedure which includes a visual check and leak testing of every tube
- Molded from virgin polypropylene under ISO certified cleanroom conditions in our UK-based production facility
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
Wells
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
- Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
- Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
- Maximum thermal conductivity for efficient heat transfer and precise thermal cycling
Frame
- Alphanumeric grid reference to aid well and sample identification
Use
- Optimized for use with the Roche LightCycler 480
- Recommended for low volume PCR
- Compatible with standard multichannel pipettes
Options
- Available with white wells give optimum signal-to-noise ratio when using fluorescent-based assays
- Combi packs available with qPCR Seal (0560)
- Available barcoded upon request; learn more about our barcoding options
- Available as a FrameStar® 2-component PCR plate with the same dimensions, but with the added benefits of a polycarbonate frame that eliminates plate distortion and warping during the PCR process, leading to less evaporation and sample loss
Specifications
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 15.60 ± 0.10mm
Well depth 15.10 ± 0.10mm
Well diameter 5.50 ± 0.10mm
Total well capacity 0.2ml (200µl)
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm
Compatibility
Roche
qPCR Cyclers (96 well block): LC96 | LC480
For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.
Ordering Information
Part Numbers
96 Well Semi-Skirted PCR Plate, Roche Style Low profile, 0.1ml wells, polypropylene, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler 480 |
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4ti-0955 | white; 50 plates |
Combi Packs | |
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4ti-0955/0560 | 4ti-0955 + qPCR Seal (4ti-0560); 50 plates + 50 seals |