Dual-Florescence for Accurate Viability Analysis of Primary Cells
AO (Acridine Orange) and DAPI (4′, 6-diamidino-2-phenylindole) are fluorescent dyes that bind to DNA, staining the nucleus of targeted cells.
AO is a permeable dye that stains the nucleus of all cells, regardless of the cell’s condition.
DAPI is an impermeable dye that only stains the nucleus of non-viable cells with damaged membranes.
To accurately evaluate the viability of cell types like PBMCs, stem cells, and splenocytes, it is advisable to use dual-fluorescence staining.
This method effectively excludes non-nucleated cells (e.g., RBCs), platelets, and debris from the samples.
AO/DAPI staining – Counting & Viability
Accurate Fluorescent Cell Counting for PBMCs
PBMCs are often mixed with RBCs or platelets. Use EVE™ HT FL to count only nucleated cells such as PBMCs.
Fluorescence based counting is more accurate than traditional Trypan Blue based counting.
More Flexible Staining Methods
EVE™ HT FL provides flexible and personalized staining choices. Cell viability can be assessed using fluorescent channels (AO/DAPI) or a bright-field channel (Trypan Blue / Erythrosine B), which meets a more diverse range of needs!
● A Growing Demand for Using Erythrosine B
Erythrosine B has the same staining principle as Trypan Blue which does not penetrate viable cells that possess intact cell membranes but only stains non-viable cells. Due to increasing environmental and health concerns, non-toxic Erythrosine B has gained attention in many European countries and is regarded as an alternative to Trypan Blue for cell viability evaluation.
Up to 48 Samples in 3 minutes
Don’t wait! Count your samples in just 3 minutes.
Leading to substantial time saving by eliminating the need for frequent reloading and waiting periods.
20 μL of sample volume
EVE™ HT FL only need 20 μL of your valuable samples.
Save your cells for more measurements or better outcome.
Level Up Your Productivity
Level up your productivity with EVE™ HT FL when compare to another cell counters.
Accurate & Precise Results You Can Rely On With Confidence
- Multi-Instrument Consistency & Precision for Cells
EVE™ HT FL instruments were put to the test using three different cell line samples (Jurkat, HeLa, and CHO) to compare their differences.
The result below showed a high instrument-to-instrument consistency with small variation.
- Excellent Linearity Across Wide Range
Following ISO standard for cell counting, we evaluated linearity of EVE™ HT FL using 2 cell lines (CHO cells and Jurkat cells). The following results demonstrate outstanding linearity.
- High correlation between EVE™ HT FL and flow cytometer
Cell samples were measured using EVE™ HT FL and a flow cytometer. For both CHO cells and PBMCs, total cell counts measured by EVE™ HT FL were highly correlated with those measured by a flow cytometer.
21 CFR Part 11 Compliance READY
EVE™ HT FL is ready for 21 CFR Part 11 compliance for cGMP facilities.
User-friendly GUI
With visualized data, the results are clear at a glance.
(Total cell, Live/Dead Cell, Viability, Cell Size)
Specifications:
- Light sources: Bright field, UV, Blue LED
- Staining dye(s): AO/DAPI mixed solution
- Analysis time: 3 ~ 18 minutes / 48 tests
- Measuring range: Detectable range: 1 x 10E4 ~ 2 x 10E7 cells/mL; Optimal range: 1 x 10E5 ~ 1 x 10E7 cells/mL: Loading volume
- 20 μL / channel
- Cell size range: Detectable size: 5 ~ 85 μm; Optimal size: 5 ~ 80 μm
- Dimensions: 586 (W) x 461 (D) x 458 (H) mm
- Weight: 61 kg
- Operation system: Windows 10
- 21 CFR Part 11 compliance Available (Optional)
