Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
Wells
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
- Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
- Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
Frame
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
Use
- Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
- Recommended for low volume PCR
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
Options
- Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
- Combi packs available with qPCR Seal (4ti-0560)
- Available barcoded upon request; learn more about our barcoding options
Specifications
FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 15.60 ± 0.25mm
Well depth 15.10 ± 0.10mm
Well diameter 5.50 ± 0.10mm
Total well capacity 0.2ml (200µl)
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm
Ct values comparison
Ct values of data run on a Roche LightCycler® 480, comparing 4titude FrameStar Roche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates
The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct value
FrameStar Roche Style, High Sensitivity Plate
Ct value: 17.23
Δ Ct: -3.05
Competitor R equivalent plates
Ct value: 20.28
Δ Ct: 0
Compatibility
ROCHE
- qPCR Cyclers (96 well block): LC96 | LC480
Ordering Information
Description
FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity Plate
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays
Part Number
4ti-0954 extra white wells, clear frame; 50 plates
Combi packs
4ti-0954/0560 4ti-0954 + qPCR Seal (4ti-0560); 50 plates + 50 seals
