Overview
We have now extended our FrameStar range to include PCR plates with optically clear flat bottom wells for microscopy applications. The FrameStar 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our Individual Access range, 4ti-0970/RA) are currently unique in the market, being suitable for use in both microscopy and PCR.
Optical bottom plates are ideal for applications requiring single cell sorting followed by molecular biology techniques such as (q)PCR and sequencing. The flat bottoms enable excellent stackability, making these plates well suited for small sample volume storage such as compound libraries, with no risk of damaging the seal of the plate below. Additionally, the small well volume enables excellent sample recovery.
Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
- Stackable
Well
- Ultra-smooth, uniform, thin-walled polypropylene wells for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.18ml (180µl) total well capacity
- Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
- Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
Frame
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
Use
- Suitable for microscopy and small volume sample storage
- Recommended for low volume PCR
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
Options
- Also available as an Individual Access plate (4ti-0970/RA) with individually detachable and sealable wells
- Available barcoded upon request; learn more about our barcoding options
Specifications
FrameStar 96 Well Skirted Optical Bottom PCR Plate
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 16.10 ± 0.25mm
Well depth 12 ± 0.10mm
Well diameter 5.46 ± 0.10mm
Total well capacity 180µl
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm
Figure 1 (click on image to expand) illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes.
The surface area of the optical base is 7.74mm2, with a thickness of 0.5mm. The total volume of a conical well is 0.2ml compared to 0.18ml for flat bottom tubes.
The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting.
Compatibility
- AGILENT/STRATAGENE
- ANALYTIK JENA/BIOMETRA
- BIO-RAD
- BIO-RAD MJ RESEARCH
- EPPENDORF
- GE HEALTHCARE/AMERSHAM
- PEQLAB/VWR
- SENSOQUEST
- TECHNE
For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table and look for the FrameStar® 96 Well Skirted PCR Plate (4ti-0960).
Ordering Information
Description
FrameStar 96 Well Skirted Optical Bottom PCR Plate
Low profile, flat optical bottom, 0.1ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100µl, total well capacity: 180µl
Part Number
4ti-0970 clear wells, clear frame; 50 plates
