a4S Automatic Roll Heat Sealer

Product Code: 4ti-0665

The perfect solution for automated heat sealing of microplates, PCR plates, assay plates and storage plates used in medium to high-throughput or full automation applications.

The main advantage of the Automated Roll Heat Sealer is that it does not require an air supply, which gives it the flexibility to be used on standard lab benches as a stand-alone unit, or integrated within a robotic set‑up. The sealer’s rapid heating element enables a fast start-up, and the stand-by function saves energy when the unit is not in use.

The Automated Roll Heat Sealer is compatible with a wide range of SBS footprint plates, including all PCR plate formats from 96 well to 1536 well, assay plates, deep well storage plates and microplates. Varying plate heights, from 3mm to 60mm can be accommodated with minimal adjustments of the instrument. Plates are presented in portrait format and the plate position on the shuttle allows good access for robotic integration.

Rolls with a width of between 75-85mm can be loaded in either of two roll positions, depending on roll length and available space, enabling the entire instrument to fit under the gantry of most liquid handling robots. The width and length of the applied seals are set to precisely cover an SBS footprint plate without interfering with plate stacking. The seal position can be adjusted to give an overhang at one end of the plate to facilitate ease of seal removal.

Support Plans

Protect the dependability of your reliable, quality instruments from Azenta Life Sciences, to ensure productivity and mitigate downtime with a Service Support Plan that suits you.

  • Corrective services
  • Remote diagnostic & repair
  • Preventative maintenance services
  • Additional services for purchase


Highest Flexibility

Compatible with a wide range of plate types, from racked tubes to 1536 well PCR plates

Flexibility of seal material choice: BioTools offers a wide range of sealing materials from Azenta Life Sciences, with a variety of properties for use with the Automated Roll Heat Sealer

Compatible with gas permeable heat seals: no instrument modification necessary for maximum convenience

Economic Efficiency


Competitive pricing: save money without compromises

Auto standby mode: saves energy and extends component life

2 year warranty on parts and labor

Extended warranty and service contracts available for peace of mind

Plug and Play Robotic Integration


The Automated Roll Heat Sealer sealer is extremely versatile leaving you the freedom to expand and configure your systems such as adding robotic plate handling

SiLA compatible: quick and easy integration with other SiLA compliant devices using the SiLA driver (available at extra cost, please see ordering information); all Azenta Life Sciences microplate sealing instruments are compliant with SiLA standards for rapid integration of automated systems

This means they can be “plug and play” connected with other instruments, such as readers, robotic arms and liquid handlers, to give a custom automated system without the need to write expensive custom drivers

Full communication protocol available: operate the instrument and record sealing conditions for each seal using the RS232 communication port



qPCR: reduce evaporation and save money

The optimized sealing performance of the Automated Roll Heat Sealer prevents reagent volume loss by evaporation

Up to a fivefold reduction in the costs of sealing can be achieved when heat sealing with the Automated Roll Heat Sealer compared to using caps or adhesive seals

Reliable qPCR results and significant reagent savings can be achieved by using Clear Heat Seal (4ti-0540) and Clear Weld Heat Seal (4ti-0573, particularly designed for hydrocyclers)

Cell assays and cell culture: increase cell viability and assay performance

Heat sealing is the optimal method for cell based assay plates and cell culture plates

It is a proven method for compound storage, particularly in protective gases such as Argon and CO₂

Gas Permeable Heat Seal (4ti-0598) is the perfect choice for cell culture: allows for optimized gaseous exchange, whilst limiting evaporation

Sample storage: strongest resistance against organic solvents

Essential requirements for long-term storage include maintaining sample integrity and minimizing the risks of sample degradation

When applied using the Automated Microplate Heat Sealer, Foil Heat Seal (4ti-0535), Pierce Heat Seal (4ti-0530), and DMSO Resistant Peel Heat Seal (4ti-0585) demonstrate the highest performance in compound storage, drug screening and subsequent sample retrieval

DMSO Resistant Peel Heat Seal is the first 100% DMSO resistant seal: pure DMSO can be stored at room temperature for 12 months with this seal with no deterioration of the seal quality

Dimensions (W x L x H): 230 x 507 x 276mm
Sealing temperature range: 100-195°C
Sealing time range: 0.1-10 sec
Weight (without roll): 27kg
Power supply V in: AC 100-240V; V out: DC 24V 320W
Power consumption: 700W (max)
Working temperature range: 10-30°C
Operating humidity (RH): 0-85%
Operating humidity (RH): 0-85%
Connection: RS-232 serial port, USB port

Ordering Information
For automated heat sealing of microplates, PCR plates, assay plates and storage plates used in medium to high-throughput or full automation applications

Part Number

4ti-0665  Includes: universal power supply, power cord, plate support adapters A (4ti-0665-2) and B (4ti-0665-3), 24 month parts and labor warranty; 1 unit Sealer adapters and accessories
4ti-0665-1    Seal Loading Tool; 1 tool
4ti-0665-2    Plate Support Adapter A, for mid height plates; 1 adapter
4ti-0665-3    Plate Support Adapter B, for 96/384 well PCR plates, 1536 well plates and standard height plates; 1 adapter
4ti-0665-56  Elevator Adapter, for very low skirted microplate; 1 adapter
4ti-0665-57  Lightweight Adapter, for low skirted microplates; 1 adapter
4ti-0665-58  Lightweight Adapter, for high skirted microplates; 1 adapter
4ti-0665-59  Lightweight Adapter, for 384 well microplates ; 1 adapter
4ti-0665-60  Lightweight Adapter, for 96 well microplates ; 1 adapter
4ti-0665-4    Roll Holder Set; includes spindle, clamping wheels, locking nut; 1 set
4ti-0665-5    Vacuum Cups, Front; clear, set of 2; 1 set
4ti-0665-6    Vacuum Cups, Back; black, set of 2; 1 set
4ti-0665-7    Plastic Tweezers; 1 tweezers
4ti-0665-8    Clear Plastic Roll Dust Cover; 1 cover
4ti-0665-41  SiLA driver; 1 license
Sealer warranties
4ti-0665-11    Warranty extension, third year (36 months total); 1 extension
4ti-0665-12    Warranty extension, third and fourth year (48 months total); 1 extension
4ti-0665-113  Warranty extension, fourth year; 1 extension

Real Time Display
  • Rapid heating element to allow for a quick start up within minutes
  • Real time temperature display to allow monitoring of heating process
Energy Saving Functions
  • When idle, unit automatically switches to standby mode: heating element temperature reduces to 60°C to save energy
  • When idle for longer periods, it automatically switches off for added safety
  • Triggering of standby and switch off modes can be adjusted to suit user’s needs
Plate Formats
  • SBS standard polypropylene and polystyrene plates and deep well blocks
  • PCR plates including skirted, semi-skirted and non-skirted formats