The Freedom Rocker™ BlotBot® is an automated blot processor for incubating and washing blotsand gels, after the transfer from gel to blot. Using a microprocessor for precise timing and dispensing, it automatically processes Westerns, Northerns, and Southerns in a consistent manner, every time. Ideal for unattended stainingof gels, it frees you from babysitting your samples. Simply add your buffers and reagents, and the BlotBot carries out your existing protocol.
- Consistent processing. Better results.
- Process blots unattended so you can focus on other tasks.
- Use as little as 3.5 mL of antibody (or up to 50 mL of reagent) for each mini blot, and recover separately.
- Large color LCD display for easy programming of protocols.
- Unattended dispensing and pouring off of liquids.
- Only requires an electrical outlet – no vacuum, pressurized air or gas, or additional pumps required.
- Can recover reagents, such as antibody solutions, separately from each gel or blot.
- Trays can hold mini blots (maximum dimension 8cm x 8cm) and midi blots (maximum dimension 8cm x 16cm) and strip blots (maximum dimension 4cm x 8cm; BlotBot 248 and 248V).
- Can switch between 2 wash buffers (BlotBot 248V only).
- Adjustable Parameters for each protocol step include: rocking speed (5 to 40 cycles per minute), reagent/solution rocking time (1 min to 96 hours), dispense volume (10 to 150 ml) up to 10 repetitions per step
- clearing rinse option
- Patented technology.
- Rocking continues after the final rinse and the wash buffer remains in the trays to prevent the blots or gels from desiccating.
- Built in the USA for years of trouble free use.
- Uses existing protocols. Programming is easy and follows standard protocols steps. Programs can be up to 9 steps where each step is an immersion in a reagent (e.g. antibody) or a series of washes.
- In each step reagents or buffer will be dispensed and aspirated to all the trays simultaneously, for consistent processing of all the blots and gels.
- The minimum volume required to fully cover each mini blot is 3.5 mL.
- A clearing rinse can be activated to provide a quick rinse to get the final drop of reagent after each wash or reagent immersion.
- Quiet.
- The removable trays can be washed. Replacements can be ordered separately.
- A bottle of buffer is connected to the Freedom Rocker BlotBot with a hose and leak-proof quick-connector connections.
- 30-day money-back guarantee.
- Two year warranty.
Throw Out Your Timers
Are you chained to your timer? Throw it out and use the Freedom Rocker BlotBot. Simply place your blots, gels, orslides in trays, set the program, connect the bottles/tubes of reagents and walk away. The microcontroller inthe Freedom Rocker BlotBot will ensure that the reagents and washes will be accurately pumped into and out ofthe trays at the precise times. Results will be consistent because all trays will receive the same reagent orwash buffer at the same time in equal volumes. Multi-dispense models can even dispense one different reagentto each tray, i.e. primary or secondary antibody.
Be productive and concentrate on another task, enjoy your lunch, OR let it run overnight!
Improve Consistency and Accuracy
By automating the processing of blots and gels, the Freedom Rocker BlotBot yields very consistent and accurate blots, gels,and slides. It automatically dispenses and aspirates equal volumes of reagents from multiple trays simultaneously. Thesamples in all the trays will experience the exact same protocol.
Shave Months Off Your Research
Complete the testing portion of your research project in half the time. Blots are ready when you arrive at work. You canreview them over coffee, not lunch, so you can start your next test iteration the same day.
Increase Productivity
Automatically processing blots and gels cuts down on distractions. Instead of babysitting your blots you have more time tofocus on other tasks, eat lunch, or attend meetings – or let it run overnight. Through increased productivity, the FreedomRocker BlotBot pays for itself in about 30 uses.
The Freedom Rocker BlotBot replicates your existing protocols. There is no need to develop new protocols so you can startprocessing right away.
Risk Free Purchase
The Freedom Rocker BlotBot comes with a 30 day money back guarantee and a 2 year warranty. Customers have been using FreedomRockers since 2005, and most of those sold in the first years remain in use today.
Use very small volumes of antibody solution
Similar to blots in a rolling bottle, a small puddle of antibody passes repeatedly over the blot in curved bottom trays.
Recover reagents separately
Reagents from each blot are captured in 50 mL tubes, ready for storage.
Use your existing protocols
Each program step matches each step in your protocol. For example, a 3X 10 min. buffer wash is a 3X 10 min. buffer wash. No development effort required.
