Low DNA Binding Products
- Azenta Life Sciences offer low binding products, recommended to support sensitive techniques such as qPCR and Next Generation Sequencing (NGS), as well as storage of samples.
- Manufactured using selected low-bind polymers, DNA loss is minimized during incubation and sample transfer, making it the ideal solution for use in applications with low DNA input.
- Low binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics. No contamination or modification of the samples.
- Tested under a broad temperature range. Maximum DNA recovery after low temperature storage and high temperature incubations.
- No detectable DNA loss during incubation steps and sample transfer in NGS sample prep and library construction. Ideally suited for sensitive applications with ultra-low nucleic acid input.
Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
Wells
- Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
- <0.2ml (200µl) working volume, 0.3ml (300µl) total well capacity
Frame
- Cut corner at A12
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
Use
- Perfect for ABI® thermal cyclers & sequencers
- Recommended for low volume PCR
- Ideal for use with robotic systems
- Compatible with standard multichannel pipettes
Options
- Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, and black
- Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays
- Also available as a clear frame with frosted wells for use with ABI®/LifeTechnologies® qPCR instruments
- Ultra-low DNA binding option available (4ti-LB0770/C) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
- Ethylene oxide treated option available (4ti-OX770C/SBC) for forensic use; learn more about our ethylene oxide treated range
- Available barcoded upon request; learn more about our barcoding options
- FrameStar 96 Lid (4ti-0289) available
Specifications
FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
Plate length 124.26 ± 0.25mm
Plate width 83.97 ± 0.25mm
Plate height 20.70 ± 0.25mm
Well depth 20.20 ± 0.10mm
Well diameter 5.46 ± 0.10mm
Total well capacity 0.3ml (300µl)
Working volume 0.2ml (200µl)
Distance to center of A1 from top edge 10.495 ± 0.25mm
Distance to center of A1 from left edge 12.63 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm
Compatibility
- ABI®/LIFE TECHNOLOGIES/THERMO FISHER SCIENTIFIC
- AGILENT/STRATAGENE
- ANALYTIK JENA/BIOMETRA
- BIOER TECHNOLOGIES
- BIO-RAD
- BIO-RAD MJ RESEARCH
- CORBETT RESEARCH
- EPPENDORF
- PEQLAB/VWR
- SENSOQUEST
- TAKARA
- TECHNE
For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.
Ordering Information
Description
FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
Standard profile, 0.2ml polypropylene wells, polycarbonate frame, cut corner A12; working volume: <200µl, total well capacity: 300µl; designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks
Part Number
4ti-0770 clear wells, purple frame; 50 plates
4ti-0770/B clear wells, blue frame; 50 plates
4ti-0770/C clear wells, clear frame; 50 plates
4ti-0770/C/10P clear wells, clear frame; 10 plates
4ti-0770/G clear wells, green frame; 50 plates
4ti-0770/R clear wells, red frame; 50 plates
4ti-0770/X clear wells, black frame; 50 plates
4ti-0771 white wells, black frame; 50 plates
4ti-0772 frosted wells, clear frame; 50 plates
4ti-0772/10P frosted wells, clear frame; 10 plates
4ti-OX770C/SBC 4ti-0770/C for forensic use, ethylene oxide treated, single barcoded; 20 plates
4ti-LB0770/C 4ti-0770/C, with low DNA binding properties; 50 plates
Lids
4ti-0289 FrameStar 96 Lid (4ti-0289), for use with the FrameStar 96 Well Semi-Skirted Plates, ABI® Style range; 50 lids
