4titude® FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style

Product Code: 4ti-0950/C

96 well semi-skirted plates designed for use on Roche LightCycler® 96 & 480 (with 96 well block). Low profile wells, especially recommended for reaction volumes <20µl. Rigid frame eliminates warping and distortion during PCR. Ideal for use with robotic systems.

  • PP wells, PC frame; cut corner H12
  • <100µl working volume, 200µl total well capacity
  • Available barcoded on request

Quality

  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
  • Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
  • Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction

Frame

  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
  • Recommended for low volume PCR
  • Ideal for use with robotic systems
  • Compatible with standard multichannel pipettes

Options

  • Standard plate comes with clear polypropylene wells on a clear polycarbonate frame
  • Also available with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Available barcoded upon request; learn more about our barcoding options
  • Combi packs available with qPCR Seal (4ti-0560) and (for 4ti-0951) with Optically Clear Windowed qPCR Seal (4ti-0564)

Specifications
FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 15.60 ± 0.25mm
Well depth 15.10 ± 0.10mm
Well diameter 5.50 ± 0.10mm
Total well capacity 0.2ml (200µl)
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm

Compatibility
ROCHE

  • qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information
Use these part numbers to request a quote, free samples, or to contact an expert:

Description
FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12; working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block)

Part Number
4ti-0950/C clear wells, clear frame; 50 plates
4ti-0951 white wells, clear frame; 50 plates

Combi packs
4ti-0952 4ti-0951 + qPCR Seal (4ti-0560); 50 plates + 50 seals
4ti-0953 4ti-0950/C + qPCR Seal (4ti-0560); 50 plates + 50 seals
4ti-0951/0565 4ti-0951 + Optically Clear Windowed qPCR Seal (4ti-0565); 50 plates + 50 seals